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| RNA Specimen Processing Frequently Asked Questions |
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| Services | RNA Specimen Processing Overview | RNA Extraction FAQ | Sample Collection and Submission Guidelines | Methods and Data Delivery | Scheduling and Shipping |
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Q: How much RNA will I get from my tissue? A: The amount of RNA extracted will depend on the tissue source, the amount of source tissue available and the care taken to prevent degradation. Cell culture: 5-10 µg/106 cells; Blood: 2-7 µg/ml blood; Tissue: 0.5-10 µg/mg tissue. Q: What starting material can I have processed for RNA extraction? A: Some of the tissues which we have extracted RNA from are: Liver, Pancreas, Brain, Spinal Cord, Enamel Organ, Nasal Punch Biopsy, Esophageal biopsy, Muscle, Cell lines, Adrenal gland, Lymphocytes, and Whole Blood. If you are planning to collect an unusual or difficult tissue type, please contact the laboratory to discuss modifications for sample collection and/or extraction. Q: Are there any special considerations when collecting samples for RNA extraction? A: RNA is easily degraded by multiple environmental factors. Please see our recommendations regarding sample collection and contact GPCL personnel for further input before beginning sample collection. Q: How is RNA quality measured? A: Concentration and purity is measured via spectrophotometry. Integrity (level of degradation) is measured using the RNA integrity number from the Agilent 2100 bioanalzyzer. See here for details. Q: What if my RNA does not pass quality measures? A: If RNA is contaminated but intact, a purification process can be performed. For degraded RNA, repeat sample collection will be necessary. Please confer with GPCL staff regarding sample collection procedures. Q: How will I get my results? A: Result files will be posted to a secure web folder. Only users registered as members of your laboratory will have access. |