You must be a registered facility user before submitting any samples for processing. If your laboratory is new to our services, please register here.
Services utilizing Capillary Electorphoresis (CE)
| | Sanger Sequencing |
| | GPCL utilizes 3730xl and 3130 DNA Analyzers from Applied Biosystems for standard Sanger sequencing. Samples may be submitted for sequencing reaction and capillary run or more experienced laboratories may submit full plates of samples for capillary run only. |
| | Genotyping Services |
| | GPCL provides support for fragment analysis technologies (AFLP and STRP genotyping) via instrument data generation only. GPCL does not perform PCR processing for these applications. PCR products can be submitted for capillary run. Instrument output data will be provided. Investigator laboratories are responsible for data analysis yielding final genotyping calls.
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CE Service Overview
Sample Preparation and Submission
Scheduling and Turn Around
Methods and Data Delivery
FAQs
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High Throughput "Next Generation" sequencing
The GPCL operates both a Roche 454 Genome Sequencer FLX and a Life Technologies SOLiD, providing support for massively parallel sequencing methodologies appropriate to a broad range of research applications. Please contact the GPCL to discuss the best approach for your research aims. High throughput sequencing is a complex technology and no samples will be accepted until an initial project intake meeting including discussion with the Bioinformatics Analysis Core has taken place.
| | 454 Sequencing
The Roche Genome Sequencer FLX, developed by 454 Life Sciences, provides more than 500 MB of sequence from one or multiple samples per run in 1,000,000 reads averaging 400 bases each. The longer reads and sequencing chemistry of this platform make it suitable for de novo sequencing, splice variant detection of transcriptomes and rare variant detection in amplicon pools among other applications.
For more information on technology and applications, please see the 454 web page.
Life Technologies SOLiD
The Life Technologies SOLiD is a "short read" instrument, currently returning up to 520 million reads per slide. Read lengths of 35 or 50 bp for either single or mate pair tags can be designated. The large number of short reads makes this platform an excellent choice for targeted and deep genome resequencing, small RNA analysis, rare variant and SNP detection and digital gene expression among other applications.
For more information on technology and applications, please see the SOLiD web page.
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