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		Research Services - DNA Sanger Sequencing Services | DNA Sequencing Services | Sanger Service Overview | FLX Service Overview | Sanger Sequencing FAQ | Sample Preparation | Methods and Data Delivery | Scheduling and Shipping   You must be a registered facility user before submitting any samples for processing. If your laboratory is new to our services, please register here. Overview The GPCL Sanger Sequencing Facility, located at 3343 Forbes Avenue in Pittsburgh, PA, runs an Applied Biosystems (ABI) 3730xl DNA Analyzer and an ABI 3130 DNA Analyzer. The Facility can be contacted at gpclseq@pitt.edu or (412) 383-9077. The Facility offers four levels of sequencing service: PCR Clean-up and Sequencing Reaction: Submit your PCR reaction for cleanup followed by sequencing reaction with your separately submitted primers or universal primers supplied by the GPCL. The GPCL will supply all materials for sequencing reactions Sequencing Reaction: Submit template and primers ready for addition of BigDye Terminator sequencing premix. Samples are cycled, dye terminators removed, and samples run on the 3730xl or 3130xl DNA Analyzer. The GPCL will supply all materials for sequencing reactions. Users need to supply DNA and primers except as noted for primer addition service. Appropriate clean up of DNA is the responsibility of the investigator Sequencing Reaction with primer addition: We can add our universal primers: M13F, M13R, SP6, T3 or T7. On the Sample drop off form, select Add Primers and indicate clearly in the space provided which samples should be treated with each primer. The GPCL will supply all materials for sequencing reactions. Appropriate clean up of DNA via column or enzymatic reaction is the responsibility of the investigator. Capillary Run: Submit your completed reactions, appropriately prepared for sequencing or genotyping reactions for analysis on the 3730xl. The Core Laboratory accepts a variety of templates, including PCR products, plasmids, BACs, etc. One sequencing run typically produces 850-950 nucleotides of high quality (98.5% accuracy) sequence data. Sample Preparation and Submission Scheduling and Turn Around Sanger Sequencing Methods and Data Delivery Tips for primer design Information on Template Preparation Sanger Sequencing FAQs