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		Research Services: SNP Genotyping Illumina® Infinium   The Infinium Assay The Infinium II Assay combines whole-genome amplification (WGA) sample preparation with direct, array-based capture and enzymatic scoring of the SNP loci. Locus discrimination is provided by a combination of sequence-specific hybridization capture and array-based, single-base primer extension. A single primer is used to interrogate a SNP locus. The 3’ end of the primer is positioned directly adjacent to the SNP site. Extension of the primer incorporates a biotin nucleotide or a dinitrophenyl labeled nucleotide. (C, G nucleotides are biotin labeled; A, T nucleotides are dinitrophenyl labeled.) Signal amplification of the incorporated label further improves the overall signal-to-noise ratio of the assay. There are multiple array formats available up to 1 million SNPs per sample. Custom iSelect panels can also be designed. See whole genome or custom content for more information on available products. Imaging and Data Analysis BeadChips are imaged using the Illumina BeadArray Reader, a twochannel, 0.8 μm resolution confocal laser scanner. The BeadStudio software package extracts whole-genome genotyping data from data files created by the Illumina BeadArray Reader. Resulting files can be exported to most standard databases and gene expression analysis programs. Sample Preparation and Submission DNA requirements vary depending on the type of Infinium array in use. Please contact GPCL for guidance in sample preparation.