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GPCL Sample Submission Guidelines for Instrument Run - Realtime PCR using Taqman Low Density Arrays
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  1. Contact GPCL to coordinate when you will bring your samples
  2. Perform your reverse transcription
  3. Mix your cDNA and master mix in a separate 1.5 ml tube for each sample.
  4. Deliver your TLDAs and sample mixes to GPCL
    1. Drop off locations are 3343 Forbes Ave Room 303, and S534A Scaife Hall
    2. Please be sure to complete a Low Density Array Drop off form. Hard copies are available in the drop off rooms.
    3. Deliver the disc that came with your TLDAs to GPCL
  5. GPCL will load your prepared cDNA+master mix onto the TLDAs that you provide and perform the run(s).
  6. Data will be available for down load from MYGPCL.
    1. Log in to MYGPCL
    2. Click on the Taqman Data link under Get Data
    3. Click on the link for the current data file.
    4. Save locally.
  7. The Bioinformatics Analysis Core is available to assist with data analysis. Contact a representative for a consultation.