Genomics Research Core

Pricing

Genomics Research Core Internal Customer Price List (USD)

1. Nucleic Acid Services

Process & Description Sample Plate Chip
1a. DNA Extraction 1-23 24-287 288+    
3ml Blood $33 $29      
6ml Blood $38 POR      
Qiamp, blood midi kit $32 $27 $22    
Oragene Saliva DNA Kit, 500ul $24 $17 $13    
High Throughput DNA Extraction     288+    
Chemagic DNA extraction buffy coat     $23    
Chemagic DNA extraction Oragene Saliva 4 ml     $18    
  1-23 24-95 96+    
DNeasy, tissue extraction $41 $35 $27    
1b. RNA Extraction 1-35 36-287 288+    
Paxgene $48 $43      
  Each (sample)    
Tempus $35    
Trizol 2-18mg tissue $35    
Trizol 500-1,000mg tissue $43    
RNeasy extraction or clean-up $36    
High Troughput RNA Extraction     288+    
Chemagic RNA exraction PAXgene / Tempus     $30    
1c. DNA/RNA Extraction Each (sample)    
All prep DNA/RNA extraction $60    
1d. Supplementary Services 1-47 48-95 96+    
Fluorometric assay, single read $3.25 $2.50 POR    
Fluorometric assay, triplicate read $8 n/a n/a    
      Each
E-gel (1-48lanes)     $69
  1-48 Each  
TapeStation RNA $8 $570  
Tapestation HS RNA or DNA $10 $720  
       
  up to 15ml blood    
PBMC Prep $43    
  Sample    
FFPE Xylene Treatment $13    

2. Sequencing (plate = 96-well)

Process & Description Sample Plate

Chip

2a. Capillary (Sanger) Sequencing; >24 samples must be in a 96-well plate   >15 per month  
price/sample
Sequencing rxn, primer and template provided $5.50  POR
  1-95 96+    
PCR Cleanup $2 $1.50    
  Partial Plate, multiples of 16 samples 1-10 per month 11-19 per month ≥20 per month  
Capillary Run $30 $150 $120 $100  
  Partial Plate, multiples of 16 samples Each  
LIZ standard added $9 $50  
2b. Next Generation Sequencing (each project is unique, please consult with Genomics Research Core staff for fees)      
Life Technologies Ion Torrent Sequencing. Resequencing, De novo sequencing, Small genome, Targeted resequencing, Whole  exome, Paired end, Fragment, RNASeq, Whole transcriptome, ChiP Seq, MeDIP POS    

3. Genotyping

Process &  Description  Sample Plate Chip
3a. Illumina Infinium: processing per sample; chip not included     <24 24-71 72+
price/sample
Illumina Infinium 8+ arrays per chip     $51 $41 $33
3b. Agena iPlex   <8 plates w/<3 plexes >8 plates w>3 plexes  
iPLEX plates/arrays, 96   $567 n/a  
iPLEX plates/arrays, 384   $1,410 $1,125  
  Each Each  
iPLEX primer pooling, per locus $8 n/a  
iPLEX multiple pools on 384 well plate per extra pool n/a $100  
Tecan rearray 96 to 384, per 96 well plate n/a $50  
3c. PCR <23 23-48 48+    
Mouse genotyping (per reaction) $10 $8.50 $5.50    
3d. Allelic Discrimination Assay <9 assays, 1 assay/plate <9 assays, multiple assays/plate Each  
TaqMan SNP $250 $310    
TaqMan SNP, plate read only   $5  

4. Expression Analysis

Process & Description Sample Plate Chip
4a. Realtime PCR (qPCR) <24 <24-48 48+    
TaqMan RT $16 $13 $10    
       
Taqman PCR Each (reaction) <4 4-10 11+  
  $6 $500 $400 $350  
    Each (96 or 384 well plate)  
Taqman Instrument Run   $30  
  Each (reaction) 1-10 arrays 11+ arrays  
SABiosciences Reverse Transcription POR n/a n/a  
Sabiosciences 384 well PCR array n/a POR POR  
4b. Affymetrix Microarray; chip not included <24 24-95 96+    
Affymetrix Whole Transcripitome Array $87 $45 $40    
WT Pico Sample Prep - PI Reagents $114 $54      
      <24 24-95 96+
Affymetrix hyb, wash, stain     $130 $100 $90

5. ddPCR

Process & Description  
Expression 8-16 (multiples of 8) 17-48 (multiples of 8) 49+ (multiples of 8)
ddPCR RNA $12.50 $8.50 $6.00
CNV      
ddPCR CNV $13.50 $9.00 $6.50
Supplementary Services < 24 Samples 24-48 Samples 48+ Samples
ddPCR RT $16 $13 $10

6. Epigenetic Analysis

Process & Description Sample Plate Chip
5a. Illumina Infinium; chip not included     <24 24-71 72+
price/sample
Infinium MethylationEPIC BeadChip     $74 $61 $52

7. NanoString (Codesets not included)

Process & Description Sample Plate Chip
6a. Expression Profiling 12-47 48-959 960+    
NanoString, mRNA $62 $58 $52    
6b. Small RNA      
NanoString miRNA $68 $62 $56    
6c. Copy number variation      
Nanostring CNV POR POR POR    

8. 10X Genomics

Process & Description  
Sample QC Sample  
Dead Cell Removal $39  
Fixed Cell Rehydration $43  
Library Preparation Base (1st sample) Samples 2-8 (each)
Single Cell Library Prep $2,800 $1,950
Linked Read Library Prep - Base (1st sample) $1,070 $375
ATAC Library Prep - Base (1st Sample) $2,890 $1,920
  Sample  
Immune Profiling (with RNA-seq) $280  
Feature Barcoding/Hash Tagging (with RNA-Seq) $320  
Sequencing (HSSC@CHP services) Flow cell  
High Output 150 cycle $3,000  
Mid Output 150 Cycle $1,350