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Welcome to the Genomics Research Core  
DNA/RNA Extraction
The specimen processing and banking laboratories have extensive RNA/DNA extraction experience for dozens of tissue types and species. Extraction can be performed in standard or high throughput formats. Whole Genome Amplification is also available. Secure specimen banking facilities are available.
-DNA
-RNA
-Bioanalyzer
-Whole Genome Amp.
DNA Analysis
We offer genetic and genomic analyses for a variety of applications. Sequencing is available in standard and high throughput formats. The choice of Taqman, Affymetrix or Illumina based SNP assay platforms is based on project goals and throughput needs.
-Sequencing
-SNP Genotyping
-DNA Methylation
-NanoString
RNA Analysis
Our MicroArray and Taqman Real Time PCR offerings allow investigators to obtain gene expression data from a flexible array of formats. Full service is available for all offerings including Affymetrix and Illumina gene expression Array products.
-Microarray Analysis
-Realtime PCR
-miRNA Analysis
-NanoString
-RNA-Sequencing
Additional Health Sciences Resource Facilities on Campus
  Biomedical Mass Spectrometry Center (BioMS)
Health Sciences Tissue Bank (HSTB)
Peptide and Peptoid Synthesis Core (Peptide)
Small Molecule Biomarker Core (SMBC)
  *Acknowledging the Genomics Research Core
All work performed in the Genomics Research Core should be acknowledged in publications and presentations.

The [name relevant portion of data] work for this publication/presentation was performed in the Genomics Research Core at the University of Pittsburgh.

It is particularly important to mention work done in the Genomics Research Core if you are making a presentation to your University of Pittsburgh colleagues.
Thank you



 
New Illumina Mouse Gene Expression BeadArrays have been discontinued.
Illumina is no longer accepting orders for the MouseWG-6 v2.0 BeadChip and the MouseRef-8 BeadChip.

The HumanHT-12 v4.0 Expression BeadChip is unaffected.

The Genomics Research Core can perform mouse gene expression microarray studies using Affymetrix 3'IVT, gene, exon, or whole transcriptome arrays. Contact us to discuss your ongoing mouse expression experiments.

The Genomics Research Core has an Ion 16S Metagenomics Kit for trial sequencing runs
Please see link for more information

Ion AmpliSeq Transcriptome Human Gene Expression Kit
Gene level expression profiling of 95% of RefSeq genes (20,800 gene targets) with 10ng total RNA input. Single tube transcriptome amplification and FFPE compatible protocol that runs on the Ion Proton at the genomics core laboratory.

The Sequenom Mass Array at the Genomics Research Core can now run 384 and 96 sample projects (link)

Lower Capillary Sanger Sequencing Prices at the Genomics Research Core!


Spring 2015 GRC Seminars

Date: Thursday, March 19, 2015
Presented by: Illumina
Title: Genotyping Array Seminar
Time: 10:00 am - 12:00 pm (noon)
Location: BST S120
Please see flyer for more information

NEWS
  • llumina GoldenGate is being phased out
    • 3,072-plex projects use the Infinium iSelect product
    • the Genomics Research Core can accommodate smaller projects on the Sequenom MassArray
    • to support ongoing projects 96-plex, 384-plex, 768-plex, and 1,536-plex GoldenGate assays will be available from Illumina through 2015
    • Mouse LD and MD Linkage Panels are no longer available
    • the Genomics Research Core can transition your mouse projects to other platforms
    Contact us for advice with transitioning GodenGate projects: 412-648-9440

  • Life Technologies Transcriptome Profiling Grant Program
    12 grants available, deadline December 12, 2014
    http://www.lifetechnologies.com/transcriptomegrant

  • Estradiol Analysis by Liquid Chromatography/Tandem Mass Spectrometry (UPLC-MS/MS)
    from Small Molecule Biomarker Core (SMBC)
    Please see link for more information

  • Pre-validated Sequenom Cancer and Methylation Panel processing is available through the Genomics and Proteomics Core Laboratories
    Please see link for more information


miRNA Posters:
miRNA realtime PCR evaluation
miRNA extraction evaluation