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Welcome to the GPCL
The GPCL is a resource of the Clinical and Translational Science Institute (ctsi). For an overview of the GPCL please see our introduction.

Bioinformatics Analysis Core
The Bioinformatics Analysis Core (BAC) of the Genomics and Proteomics Core Laboratories provides data analysis services for a broad range of data types. We are available to consult and participate in basic and clinical study design, including patient accrual algorithms and multivariable case/control selection.
DNA/RNA Extraction
The specimen processing and banking laboratories have extensive RNA/DNA extraction experience for dozens of tissue types and species. Extraction can be performed in standard or high throughput formats. Whole Genome Amplification is also available. Secure specimen banking facilities are available.
-Whole Genome Amp.
DNA Analysis
We offer genetic and genomic analyses for a variety of applications. Sequencing is available in standard and high throughput formats. The choice of Taqman, Affymetrix or Illumina based SNP assay platforms is based on project goals and throughput needs.
-SNP Genotyping
-DNA Methylation
RNA Analysis
Our MicroArray and Taqman Real Time PCR offerings allow investigators to obtain gene expression data from a flexible array of formats. Full service is available for all offerings including Affymetrix and Illumina gene expression Array products.
-Microarray Analysis
-Realtime PCR
-miRNA Analysis
  Biomedical Mass Spectrometry Center
Proteomics services are now offered through the Biomedical Mass Spectrometry Center (BioMS Center), a campus wide shared facility that brings together UPCI and Office of Research Health Sciences staff and resources to advance the application of mass spectrometry in basic and translational research. The Center houses eleven modern instruments, including three hybrid Orbitrap Fourier-transform mass spectrometers that enable state-of-the-art structural characterization and quantification of biologically relevant proteins. The Center is open to researchers that are looking to have experiments performed by staff scientists, as well as researchers that want to take a "hands on" approach and collect their own data. The capabilities of the center include: 1) the identification of proteins from polyacrylamide gels, 2) targeted proteomics, and 3) discovery proteomics.
Affiliated Services

Peptide Synthesis Core
The Peptide Synthesis Core provides accessible, economical, comprehensive, and state-of-the-art synthetic peptide production services. These services include peptide design, synthesis, purification, and quality control, including certified peptides for use in phase I and phase II clinical trials.
Additional CTSI Resource Facilities on Campus
  Small Molecule Biomarker Core (SMBC)
Health Sciences Tissue Bank (HSTB)
Stem Cell Core (SCC)
Pitt Innovation Challenge
Take the Pitt Innovation Challenge (PInCh), see:
Awards ranging from $25K to $100K
Deadline: Sep. 15, 2014

The Sequenom Mass Array at the GPCL can now run 384 and 96 sample projects (link)

The GPCL NanoString Syatem supports nCounter Elements codesets (link)

New! Illunina SNP genotyping array pricing:
Illumina has significantly reduced the cost of off-the-shelf Infinium SNP genotyping arrays. The GPCL can process all Infinium arrays using a robust, efficient and cost-effective automated workflow.

New!Ion Torrent NextGen Sequencing Services now available at the GPCL

New!Sample pickup at Technology drive

Genome wide and custom targeted Expression Analysis Services offered by the GPCL

GPCL supports SNP Platforms for User-Defined Content for targeted analysis and Fixed Content for mapping and association

NanoString Now available at the GPCL for Digital mRNA, miRNA, Inc RNA and CNV profiling!

Lower Capillary Sanger Sequencing Prices at the GPCL!

  • Estradiol Analysis by Liquid Chromatography/Tandem Mass Spectrometry (UPLC-MS/MS)
    from Small Molecule Biomarker Core (SMBC)
    Please see link for more information

  • Pre-validated Sequenom Cancer and Methylation Panel processing is available through the Genomics and Proteomics Core Laboratories
    Please see link for more information

  • Genomics Services for Massively Parallel Sequencing
    Please see flyer for more information

  • LTQ Velos Orbitrap (Thermo) now operational in the GPCL Proteomics Laboratory, call 412-383-5937 for details.

  • Mass spectrometry-based proteomic analysis services
    Please see flyer for more information

GPCL BAC Announcement:
The Clinical and Translational Science Institute and the Office of Research, Health Sciences are pleased to announce the formation of the Bioinformatics Analysis Core within the GPCL.
Read more

miRNA Posters:
miRNA realtime PCR evaluation
miRNA extraction evaluation